Thermo Fisher Scientific MEK1 Polyclonal Antibody
MEK1 단백질을 표적으로 하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, IP 등에 사용 가능하며 Human에 반응. 항원 친화 크로마토그래피로 정제, 1 mg/mL 농도, 4°C 보관.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 343 and 393 of human MAPK/ERK kinase 1 (Dual specificity mitogen-activated protein kinase kinase 1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt.
- For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
- Based on 100% sequence identity, this antibody is predicted to react with X. laevis, Rabbit, and Chimpanzee.
Target Information
MAP2K1 (MEK1) kinase is a dual-specificity protein kinase functioning in mitogen-activated protein kinase (MAPK) cascades, controlling cell growth and differentiation.
It is activated by various growth factors, cytokines, membrane depolarization, and calcium influx.
MEK1 activation occurs via phosphorylation of serine residues 218 and 222 by Raf1.
It is involved in stress response, apoptosis, and cytokine-induced proliferation.
Mutations can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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