Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Ultra Violet 805, eBioscience

Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Ultra Violet 805, eBioscience

Applications

• Flow Cytometry (Intracellular Staining)

Tested Dilution: ≤ 1.0 µg/test

Product Specifications

Additional Formats Available

• Alexa Fluor 488
• Alexa Fluor 700
• APC
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 711
• eFluor 450
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1).
Compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 1.0 µg per test (100 µL final volume).

Brilliant Ultra Violet™ 805 (BUV805):

• Tandem dye emitting at 797 nm
• Requires UV laser (355 nm) detection capability
• Sensitive to light; protect vial and samples from light

Fixation Compatibility:

• Stable up to 3 days at 4°C in IC Fixation Buffer or 1-step Fix/Lyse Solution
• Compatible with short-term methanol fixation (≤ 1 hour)

Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet

BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It regulates nitric oxide production by depriving NO synthase of arginine substrate.
Expressed in liver, neutrophils, myeloid-derived suppressor cells, and neural stem cells.
In mice, Arginase 1 marks alternatively activated macrophages (M2a).
Defects cause argininemia, an autosomal recessive disorder with hyperammonemia.

For Research Use Only. Not for diagnostic procedures or resale without authorization.

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