Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Ultra Violet 805, eBioscience
인간 및 마우스 Arginase 1을 인식하는 단클론 항체로, Brilliant Ultra Violet 805 형광체로 결합되어 세포 내 염색 및 유세포분석에 적합. UV 레이저(355 nm) 사용 장비에 최적화되어 있으며, 광민감성 주의 필요. 연구용으로만 사용.
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Thermo Fisher Scientific Arginase 1 Monoclonal Antibody (A1exF5), Brilliant Ultra Violet 805, eBioscience
Applications
- Flow Cytometry (Intracellular Staining)
Tested Dilution: ≤ 1.0 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Brilliant Ultra Violet™ 805 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A1exF5 |
| Immunogen | E.coli-derived recombinant mouse Arginase 1 |
| Conjugate | Brilliant Ultra Violet™ 805 |
| Excitation / Emission Max | 349 / 804 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2896141 |
Additional Formats Available
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- Brilliant Violet 421
- Brilliant Violet 480
- Brilliant Violet 650
- Brilliant Violet 711
- eFluor 450
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1).
Compatible with standard intracellular staining protocols and the Foxp3/Transcription Factor Staining Buffer Set.
Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Tested on normal human peripheral blood cells using Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00). Recommended ≤ 1.0 µg per test (100 µL final volume).
Brilliant Ultra Violet™ 805 (BUV805):
- Tandem dye emitting at 797 nm
- Requires UV laser (355 nm) detection capability
- Sensitive to light; protect vial and samples from light
Fixation Compatibility:
- Stable up to 3 days at 4°C in IC Fixation Buffer or 1-step Fix/Lyse Solution
- Compatible with short-term methanol fixation (≤ 1 hour)
Excitation: 355 nm
Emission: 797 nm
Laser: Ultraviolet
BRILLIANT ULTRA VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, the final step in the urea cycle.
It regulates nitric oxide production by depriving NO synthase of arginine substrate.
Expressed in liver, neutrophils, myeloid-derived suppressor cells, and neural stem cells.
In mice, Arginase 1 marks alternatively activated macrophages (M2a).
Defects cause argininemia, an autosomal recessive disorder with hyperammonemia.
For Research Use Only. Not for diagnostic procedures or resale without authorization.
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