Thermo Fisher Scientific SOX2 Monoclonal Antibody (20G5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:2,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 18 publications 18 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

View 9 publications 9 publications

Flow Cytometry (Flow)

1:100

-

Immunoprecipitation (IP)

5 µg

-

ChIP assay (ChIP)

1-3 µL

-

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Dog, Fish, Human, Mouse

Published species

Bovine, Dog, Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

20G5

Immunogen

Full-length human recombinant protein expressed in bacteria if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:SOX2,uniProtId:P48431-1,ncbiNodeId:9606,antigenRange:1-317,antigenLength:317,antigenImageFileName:MA1-014_SOX2_P48431-1_House_mouse.svg,antigenImageFileNamePDP:MA1-014_SOX2_P48431-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• DyLight 488
• DyLight 550
• DyLight 650
• HRP
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with 30% glycerol, 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536667

Product Specific Information

Western blot analysis of MA1-014 specifically detects SOX2 protein at ~36 kDa in the nucleus of human and mouse embryonal carcinoma and iPS cells.

Target Information

SOX2 is an intronless gene encoding a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of the SOX2 gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. The SOX2 gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). Further, SOX2 protein may act as a transcriptional activator after forming a protein complex with other proteins. Mutations in the SOX2 gene have been associated with bilateral anophthalmia, a severe form of structural eye malformation, optic nerve hypoplasia and syndromic microphthalmia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.