Thermo Fisher Scientific TRAP1 Monoclonal Antibody (TRAP1-6)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:100

-

Immunohistochemistry (Frozen) (IHC (F))

1:20

-

Immunocytochemistry (ICC/IF)

1:250

-

Flow Cytometry (Flow)

-

View 1 publication 1 publication

Immunoprecipitation (IP)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

TRAP1-6

Immunogen

Purified recombinant TRAP1.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

purified

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2207987

Product Specific Information

MA1-010 detects tumor necrosis factor receptor-associated protein (TRAP1) from human tissues.

MA1-010 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~75 kDa protein representing TRAP1. Immunofluorescence staining of TRAP1 in PC-3-M cells with MA1-010 produces a pattern consistent with mitochondrial staining. Immunoprecipitation of TRAP1 using MA1-010 fails to co-precipitate p23, Hop, or CyP40 suggesting TRAP1`s inability to associate with these co-chaperones.

The MA1-010 immunogen is purified, recombinant, human TRAP1.

Target Information

The 90 kDa heat shock protein (hsp90) family of molecular chaperones is a highly conserved family of proteins that play an important physiological role. Hsp90 is involved in numerous cellular processes but is best known for its association with signal transduction machinery. A recently cloned homolog of hsp90 is the tumor necrosis factor receptor-associated protein (TRAP1). Like hsp90, TRAP1 is found to be associated with numerous proteins involved in diverse actions. Immunofluorescence data has shown TRAP1 to be localized in the mitochondria of mammalian cells. This observation and the fact that TRAP1 is shown to have a mitochondrial targeting presequence strongly implicates TRAP1 as a mitochondrial matrix protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.