Thermo Fisher Scientific Phospho-Girdin (Ser1416) Polyclonal Antibody

Applications

• Western Blot (WB)
  Tested Dilution: 1:1,000

Product Specifications

Product Specific Information

The antibody was cross-adsorbed to unphosphorylated Girdin (Ser-1416) peptide before affinity purification using phospho-Girdin (Ser-1416) peptide.
This antibody detects a 250 kDa* protein on SDS-PAGE immunoblots of human A431 cells stimulated with EGF or calyculin A.
These reactivities are not observed after lambda phosphatase treatment to dephosphorylate Girdin.

Target Information

Girdin is a Galpha-interacting protein that can enhance the activation of the protein kinase Akt, remodel the actin cytoskeleton, and is thought to be involved in the regulation of cell migration and cancer metastasis.
It has recently been shown that Girdin interacts with Disrupted-in-Schizophrenia 1 (DISC1), a susceptibility gene for major psychiatric disorders.
DISC1 is thought to be involved in the migration, positioning and differentiation of dentate granule cells (DGCs) during development.
Depletion of Girdin or blocking the Girdin-DISC1 interaction results in defects in axonal sprouting in the CA3 region of the hippocampus and overextended migration or mispositioning of DGCs, suggesting that Girdin plays a role in postnatal neurogenesis in the dentate gyrus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.