Thermo Fisher Scientific Phospho-Estrogen Receptor alpha (Tyr537) Polyclonal Antibody

Applications

• Western Blot (WB)
  Tested Dilution: 1:100

Product Specifications

Product Specific Information

The antibody was cross-adsorbed to unphosphorylated ERα (Tyr-537) peptide before affinity purification using phospho-ERα (Tyr-537) peptide (without carrier).
This antibody detects several forms of ERα ranging from 66 to 35 kDa on SDS-PAGE immunoblots of MCF-7 cells treated with pervanadate, and this reactivity is removed after alkaline phosphatase treatment.

Target Information

Estrogen Receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptors.
The estrogen receptor is a ligand-activated transcription factor that, when bound to estrogen hormone, induces a conformational change allowing dimerization and binding to estrogen response elements (ERE) in DNA.
When bound to EREs, ER can positively or negatively regulate gene transcription through recruitment of coactivator or corepressor proteins.

There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2).
Due to alternative RNA splicing, at least four estrogen receptor-alpha isoforms are known to exist (Isoform 1: 66 kDa, Isoform 2: 53 kDa, Isoform 3: 47 kDa, Isoform 4: 35 kDa).
Estrogen receptors are widely expressed in various tissues and are essential for sexual development and reproductive function, as well as playing roles in bone and other tissues.
They are implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.