Thermo Fisher Scientific NGEF Polyclonal Antibody
NGEF 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot과 ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. Ephexin-1 C-말단 합성 펩타이드로 면역화. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Ephexin-1 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse ephexin-1. This sequence has significant homology to the same region in human and rat ephexin-1, and has low homology to other known proteins. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Wet ice |
| RRID | AB_2942924 |
Product Specific Information
The antibody detects an 80 kDa band corresponding to ephexin-1 in Western blots of human A431, Jurkat, and HUVEC cells, as well as rat PC12 cells and mouse brain tissue.
Target Information
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1, and CDC42. Plays a role in axon guidance by regulating ephrin-induced growth cone collapse and dendritic spine morphogenesis. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA, resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1- and CDC42-stimulated growth cone extension.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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