Thermo Fisher Scientific NF-Y Polyclonal Antibody
NF-Y 단백질을 인식하는 Rabbit Polyclonal Antibody로 인간 시료에 반응. Western blot 및 ELISA에 적합. 고순도 이온 교환 크로마토그래피로 정제된 액상 형태. 장기 보관 시 -20°C에서 안정적.
- 카탈로그번호
- 200-401-100
- 판매단위
- pk
카탈로그
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
ELISA
- Tested Dilution: 1:10,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Anti-NF-Y (A) antibody was prepared from monospecific antiserum by a multi-step procedure that includes delipidation, salt fractionation, and ion exchange chromatography. A single precipitin arc was observed against anti-Rabbit Serum when assayed by immunoelectrophoresis.
Target Information
The SMN1 gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions.
The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region.
The telomeric and centromeric copies of this gene are nearly identical and encode the same protein — survival motor neuron protein.
The SMN complex plays a catalyst role in the assembly of small nuclear ribonucleoproteins, the building blocks of the spliceosome.
Mutations in the SMN1 gene are known to cause spinal muscular atrophy types 1 and 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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