Thermo Fisher Scientific ETV4 Polyclonal Antibody
ETV4 단백질을 인식하는 Thermo Fisher Scientific의 고순도 Rabbit Polyclonal Antibody. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. 인간, 마우스, 랫트 반응성. 항원 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full length Human ETV4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2720552 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
Target Information
ETV4 (E1A enhancer binding protein, E1AF), also known as PEA3, is part of the Ets gene family encoding sequence-specific DNA binding proteins. Members include PU.1, Ets-1, Ets-2, E74, and GABP-a. These proteins recognize DNA motifs containing a central 5'-GGAA-3' element.
PEA3 binds the motif 5'-AGGAAG-3' but not 5'-AGGAAC-3', showing unique flanking sequence recognition.
PEA3 is expressed in epithelial and fibroblastic cells but not in hematopoietic cells, contrasting with Ets-1, Ets-2, and Fli-1, which are primarily expressed in hematopoietic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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