Thermo Fisher Scientific GCN5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human KAT2A (NP_0665642) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:GCN5,uniProtId:Q92830-1,ncbiNodeId:9606,antigenRange:1-100,antigenLength:837,antigenImageFileName:PA5-116963_GCN5_Q92830-1_Rabbit.svg,antigenImageFileNamePDP:PA5-116963_GCN5_Q92830-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.06 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901593

Product Specific Information

Positive Samples: HeLa, NIH/3T3, C6

Immunogen sequence: MAEPSQAPTP APAAQPRPLQ SPAPAPTPTP APSPASAPIP TPTPAPAPAP AAAPAGSTGT GGPGVGSGGA GSGGDPARPG LSQQQRASQR KAQVRGLPRA

Target Information

SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group. Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.