Thermo Fisher Scientific H3K27me1 Polyclonal Antibody
H3K27me1 단일메틸화 히스톤 H3를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA, ChIP 등 다양한 응용 가능. Human, Mouse, Rat 반응성. Affinity purified, PBS/glycerol buffer, -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| ChIP assay (ChIP) | 5 µg antibody for 5–10 µg of chromatin |
| Dot blot (DB) | 1:500–1:2,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic monomethylated peptide around K27 of human histone H3 (NP_003520.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.48 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901595 |
Product Specific Information
- Positive samples: HeLa, NIH/3T3, C6
- Immunogen sequence: ARKSA
Target Information
Histone H3 is a DNA-binding protein found in the chromatin of all eukaryotic cells. Together with other core histones, it binds DNA to form the nucleosome structure. Histones play a central role in transcription regulation, DNA repair, replication, and chromosomal stability. Post-translational modifications of histones, such as methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine, influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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