Thermo Fisher Scientific S100A10 Monoclonal Antibody (344015)
S100A10 단백질을 인식하는 Rat IgG2a 단클론 항체로, Western blot, ICC/IF, Flow cytometry에 적합. 인간 및 마우스 시료 반응성. 단백질 A/G로 정제된 동결건조 형태. PBS 및 trehalose 완충액에 재구성 가능. 연구용 전용 시약.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5–25 µg/mL |
| Flow Cytometry (Flow) | 0.25 µg per million cells |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rat / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 344015 |
| Immunogen | E. coli-derived recombinant mouse S100A10 (Met1–Lys97) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2609624 |
Product Specific Information
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse S100A4, S100A8, S100A9, recombinant human S100B, or S100P is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
The S-100 family of calcium-activated proteins interact with a range of target proteins to modulate biological signaling pathways.
Numerous cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular cell surface, where it forms a heterotetrameric complex with Annexin II. This association is not required for plasma membrane localization or for binding and activation of plasminogen.
Additionally, S-100A10 acts as a cellular chaperone for hepatitis B virus polymerase. Normally localized in the cytoplasm, the polymerase relocates partially to the nucleus in the presence of S-100A10, suggesting a role for S-100A10 and intracellular calcium in viral replication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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