Thermo Fisher Scientific S100A10 Monoclonal Antibody (344015)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunocytochemistry (ICC/IF)

5-25 µg/mL

-

Flow Cytometry (Flow)

0.25 µg per million cells

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

344015

Immunogen

E. coli-derived recombinant mouse S100A10 Met1-Lys97 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:S100A10,uniProtId:P08207-1,ncbiNodeId:10090,antigenRange:1-97,antigenLength:97,antigenImageFileName:MA5-24080_S100A10_P08207-1_Rat.svg,antigenImageFileNamePDP:MA5-24080_S100A10_P08207-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2609624

Product Specific Information

In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse (rm) S100A4, rmS100A8, rmS100A9, recombinant human (rh) S100B, or rhS100P is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

The S-100 family of calcium-activated proteins interact with a range of target proteins to modulate biological signaling pathways. Numerous cancer cell lines overexpress the plasminogen receptor S-100A10 on the extracellular cell surface, where it forms a heterotetrameric complex with Annexin II, though this association is not required for plasma membrane localization or binding and activation of plasminogen. Additionally, S-100A10 acts as a cellular chaperone for hepatitis B (Hep B) virus polymerase. Hep B virus polymerase normally localizes to the cytoplasm only, though in the presence of S-100A10 a portion relocates to the nucleus, implying a role for S-100A10 and intracellular calcium in the process of viral replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.