Thermo Fisher Scientific GUCY1B3 Recombinant Rabbit Monoclonal Antibody (ARC2071)
GUCY1B3 단백질을 인식하는 토끼 단클론 항체로, Western blot, IHC, ICC, ELISA 등 다양한 응용에 적합. HEK293 세포에서 발현된 재조합 항체이며, 높은 특이성과 재현성을 제공. PBS 버퍼 내 50% 글리세롤로 안정화되어 장기 보관 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:6,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:800 |
| Immunocytochemistry (ICC/IF) | 1:100–1:400 |
| ELISA | 1 µg/mL |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC2071 |
| Immunogen | Synthetic peptide corresponding to amino acids 1–100 of human Guanylyl Cyclase beta 1 (GUCY1B3) (UniProt ID: Q02153) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS (pH 7.3) with 50% glycerol, 0.05% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898004 |
Product Specific Information
- Positive Samples: U-87MG, Mouse lung, Mouse brain, Mouse heart, Rat brain
- Immunogen Sequence:
MYGFVNHALE LLVIRNYGPE VWEDIKKEAQ LDEEGQFLVR IIYDDSKTYD LVAAASKVLN LNAGEILQMF GKMFFVFCQE SGYDTILRVL GSNVREFLQN
Target Information
Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase: a soluble form (GCS or sGC), which acts as a receptor for nitric oxide, and a membrane-bound receptor form (GC), which serves as a peptide hormone receptor.
The GC-C protein consists of an extracellular domain, a single transmembrane domain, and a cytoplasmic region with kinase-like and catalytic domains. It exists as two glycosylated forms: a 130 kDa precursor in the endoplasmic reticulum and a 145 kDa form on the plasma membrane. Ligand binding to GC-C promotes cGMP accumulation.
GC-C acts as a receptor for heat-stable enterotoxins from pathogenic E. coli and also binds guanylin and uroguanylin peptides, which regulate renal function in response to salt intake.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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