Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), NovaFluor Red 660, eBioscience

Applications

• Flow Cytometry (Flow): 4 µL (0.6 µg)/test

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Product Specifications

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Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 561, 660, 700
• APC, APC-eFluor 780
• Biotin, Brilliant UV/Violet Series
• eFluor 450, 506
• FITC, PE, PE-Cyanine5, PE-Cyanine7
• Super Bright Series
• NovaFluor Blue, Red, Violet, Yellow Series
• Request custom conjugation available

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Product Specific Information

Description:
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR with immobilized UCHT1 induces intracellular signaling leading to T cell activation and proliferation.

Applications Reported:

• Flow cytometric analysis

Applications Tested:

• Pre-titrated and validated for flow cytometric analysis of normal human peripheral blood cells
• Recommended use: 4 µL (0.6 µg) per 100 µL test volume
• Suitable for 10⁵–10⁸ cells/test

NovaFluor Compatibility Note:
NovaFluor dyes are not compatible with DNA intercalating viability dyes such as propidium iodide, 7-AAD, and DAPI.
Use Invitrogen LIVE/DEAD Fixable Dead Cell stains instead.

Staining Recommendations:
Each NovaFluor conjugate or kit is shipped with CellBlox Blocking Buffer.

• Add 5 µL per sample when staining with NovaFluor conjugates
• For single-color controls: add 5 µL per 100 µL of cell sample (10³–10⁸ cells)

Excitation/Emission:

• Excitation: 637 nm
• Emission: 659 nm
• Laser: 633–640 nm (Red)

Technology:
NovaFluor conjugates use Phiton™ technology, employing nucleic acid dye structures engineered for optimized fluorescence signatures with reduced spillover and spread.

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Target Information

The CD3 subunit complex transduces antigen-recognition signals into T cell cytoplasm and regulates TCR surface expression.
The complex includes CD3 gamma, delta, epsilon, and zeta subunits, all members of the immunoglobulin superfamily encoded on human chromosome 11.

Biological Roles:

• Mediates TCR-induced activation, proliferation, and survival
• Present on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes
• Absent on B and NK cells
• CD3 gene defects are associated with CD3 immunodeficiency

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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