Thermo Fisher Scientific RAB5A Polyclonal Antibody
RAB5A 단백질을 인식하는 Sheep 유래 Polyclonal 항체로, Western blot과 IHC에 사용 가능. Mouse와 Rat에 반응하며, PBS 내 동결건조 형태로 제공. 세포 내 막 수송 및 엔도솜 융합 연구에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 10–50 µg/mL | – |
| Immunohistochemistry (IHC) | 10–50 µg/mL | – |
| Miscellaneous PubMed (Misc) | – | View 2 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the internal region of mouse Ras-related protein Rab-5A (RAB5A) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not Determined |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.
- The antigen is homologous in rat.
- Specificity: RAB5A.
Target Information
RAB5 proteins are small GTPases that regulate intracellular membrane trafficking, from vesicle formation to membrane fusion. They cycle between inactive GDP-bound and active GTP-bound forms, recruiting downstream effectors responsible for vesicle formation, movement, tethering, and fusion.
RAB5A is essential for plasma membrane and early endosome fusion, regulates filopodia extension, and is required for exosomal release of SDCBP, CD63, PDCD6IP, and syndecan. It also regulates maturation of apoptotic cell-containing phagosomes, likely downstream of DYN2 and PIK3C3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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