Thermo Fisher Scientific CD15 Monoclonal Antibody (HI98), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)
  • Tested Dilution: 5 µL (0.25 µg)/test
  • View 2 publications

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• eFluor 615
• FITC
• PE
• PE-Cyanine7
• Request custom conjugation

Product Specific Information

Description:
The HI98 monoclonal antibody reacts with human CD15 (Lewis X). This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes; monocytes express it at varying degrees, while lymphocytes are negative.

Applications Reported:
This HI98 antibody has been reported for use in flow cytometric analysis.

Applications Tested:
Pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Use at 5 µL (0.25 µg) per test.
A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL.
Cell number should be determined empirically (10⁵–10⁸ cells/test).

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm).
It can be used in place of PerCP-Cyanine5.5.
Recommended filter: 710/50 bandpass (695/40 acceptable).
Ensure your instrument supports this fluorochrome.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
  (100 µL cell sample + 100 µL buffer)
• 1-step Fix/Lyse Solution (Product #00-5333-54)

Storage up to 3 days in the dark at 4°C with minimal brightness loss.
Clone-specific performance should be determined empirically.

Excitation / Emission / Laser:
488 nm / 710 nm / Blue Laser

Filtration:
0.2 µm post-manufacturing filtered.

Target Information

CD15 (Lewis X, Le(x); SSEA-1):
A trisaccharide determinant (3-fucosyl-N-acetyllactosamine) expressed on glycolipids, glycoproteins, and proteoglycans of various cell types such as granulocytes, mast cells, monocytes, macrophages, gastric mucosa, nervous system, and tumor cells.
Variants include sialyl-Lewis X and sulphated Lewis X.
Cells with high Le(x) expression show strong calcium-dependent self-aggregation, involved in embryo compaction and teratocarcinoma cell aggregation.
Sialyl-Le(x) and sialyl-Le(a) act as selectin ligands.
CD15 expression is widely used to confirm Hodgkin’s disease diagnosis.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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