Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PerCP-eFluor 710, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (eBioCB16 (CB16)), PerCP-eFluor 710, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.06 µg)/test
Publications: View 22 publications

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 661 / 737 / 805
• Brilliant Violet 480
• eFluor 450 / 506
• FITC
• Functional Grade
• NovaFluor Blue 610-70S / 660-120S
• NovaFluor Red 710 / 725
• NovaFluor Yellow 660 / 730
• PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG (50–80 kDa).
CD16 includes two genes:

• CD16A (FcγRIIIA): expressed in macrophages and NK cells (polypeptide-anchored)
• CD16B (FcγRIIIB): expressed in neutrophils (GPI-anchored)

Two polymorphisms (NA-1, NA-2) exist in CD16B; NA-2 homozygotes show lower phagocytic capacity.
CD16 binds IgG immune complexes, preferentially IgG1 and IgG3, initiating signal transduction cascades that lead to:

• Antibody-dependent cell-mediated cytotoxicity (ADCC)
• Phagocytosis
• Degranulation
• Proliferation

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Pre-titrated and validated using normal human peripheral blood cells.
Use at 5 µL (0.06 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test).

Fluorochrome Information:
PerCP-eFluor® 710 emits at 710 nm, excited by 488 nm (blue laser).
Recommended filter: 710/50 bandpass (695/40 acceptable alternative).
Light-sensitive; protect from light.

Fixation:
Samples can be stored up to 3 days in the dark at 4°C using:

• IC Fixation Buffer (Product #00-8222)
• 1-step Fix/Lyse Solution (Product #00-5333)
  Minimal impact on brightness and FRET efficiency.

Excitation / Emission / Laser: 488 nm / 710 nm / Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD16 (FCGR3A) encodes a receptor that recognizes the Fc portion of IgG, mediating:

• Clearance of immune complexes
• Cell-mediated cytotoxicity
• Enhancement of viral infection

Gene Information:

• FCGR3A and FCGR3B share high similarity (chromosome 1)
• FCGR3A: expressed in NK cells (transmembrane anchoring)
• FCGR3B: expressed in neutrophils (PI linkage)

Clinical Relevance:
Mutations linked to immunodeficiency 20, systemic lupus erythematosus, recurrent viral infections, and alloimmune neonatal neutropenia.
Alternative splicing produces multiple isoforms.

For Research Use Only. Not for diagnostic or resale purposes.

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