Thermo Fisher Scientific Strep Tag II Monoclonal Antibody (A5D10)
Strep-tag II 서열을 인식하는 단클론 항체로, Western blot 등 단백질 검출에 적합합니다. 마우스 IgG1 형식이며 비결합(Unconjugated) 상태로 제공됩니다. Protein G로 정제되어 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications
- Western Blot (WB): 1:1,000–1:5,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | A5D10 |
| Immunogen | Synthetic peptide corresponding to Strep-tag II |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Protein G |
| Storage Buffer | TBS, pH 7.4, with 0.2% BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2931444 |
Target Information
The Strep-tag II is an eight-residue minimal peptide sequence (Trp-Ser-His-Pro-Gln-Phe-Glu-Lys) that exhibits intrinsic affinity toward streptavidin and can be fused to recombinant proteins in various fashions.
The original Strep-tag was a nine amino acid peptide with high specificity and affinity toward streptavidin, allowing simple purification of proteins by affinity chromatography, but it could only be attached to the C-terminus of recombinant proteins.
To enable placement at the N-terminus as well, it was re-engineered and renamed Strep-tag II.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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