Thermo Fisher Scientific MEK5 Polyclonal Antibody
MEK5 단백질을 인식하는 Thermo Fisher Scientific의 rabbit polyclonal 항체. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용 가능. 인간, 마우스, 랫트 반응성. 고순도 Affinity Chromatography 정제, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–190 of human MEK5 (NP_660143.1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 2.95 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914620 |
Product Specific Information
Positive test controls include:
- NCI-H460
- BT-474
- Mouse liver
- Rat liver
- Rat kidney
Immunogen sequence:
MLWLALGPFP AMENQVLVIR IKIPNSGAVD WTVHSGPQLL FRDVLDVIGQ VLPEATTTAF EYEDEDGDRI TVRSDEEMKA MLSYYYSTVM EQQVNGQLIE PLQIFPRACK PPGERNIHGL KVNTRAGPSQ HSSPAVSDSL PSNSLKKSSA ELKKILANGQ MNEQDIRYRD TLGHGNGGTV YKAYHVPSGK
Target Information
MAP2K5 (MEK5) is a serine/threonine kinase belonging to the MAP kinase family. It specifically interacts with and activates MAPK7/ERK5. MAP2K5 can be phosphorylated and activated by MAP3K3/MEKK3 and atypical protein kinase C isoforms (aPKCs).
This signaling cascade is involved in growth factor–stimulated cell proliferation and muscle cell differentiation.
The rs3743354 polymorphism in the MAP2K5 promoter may influence colorectal cancer (CRC) risk.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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