Thermo Fisher Scientific BAG4 Polyclonal Antibody
Western blot 및 ICC/IF에 적합한 BAG4 폴리클로날 항체로, Rabbit IgG 호스트에서 생산됨. Human, Mouse, Rat 반응성. PBS에 0.02% sodium azide 포함된 액상 형태. 냉장 보관(2-8°C) 권장, 장기 보관 시 -20°C. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to amino acids near the amino terminus of human SODD (BAG4, UniProt ID: O95429-1, antigen length: 457 aa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152812 |
Product Specific Information
- In Western blot applications, this antibody detects a band at approximately 60 kDa.
- A suggested positive control is HeLa cell lysate.
- PA5-19925 can be used with blocking peptide PEP-0050.
Target Information
The protein encoded by this gene is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with various apoptosis and growth-related proteins, including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors, and members of the heat shock protein 70 kDa family.
This protein contains a BAG domain near the C-terminus, which can bind and inhibit the chaperone activity of Hsc70/Hsp70. It is associated with the death domain of TNF-R1 and DR3, negatively regulating downstream cell death signaling. Its regulatory role in apoptosis has been demonstrated in epithelial cells that undergo apoptosis upon loss of integrin-mediated matrix contacts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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