Thermo Fisher Scientific Phospho-STAT1 (Tyr701) Monoclonal Antibody (S.213.5)
Phospho-STAT1 (Tyr701) 단일클론 항체로 인간 및 마우스 시료에 반응하며, WB, IHC, ICC, Flow, IP, ChIP 등 다양한 응용에 사용 가능. 다른 Stat 단백질의 인산화 타이로신과 교차반응 없음. 고순도 친화크로마토그래피 정제, 액상 형태로 -20°C 보관.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400–1:1,600 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:800 | View 2 publications |
| Flow Cytometry (Flow) | 1:100–1:400 | - |
| Immunoprecipitation (IP) | 1:100 | - |
| ChIP assay (ChIP) | 1:100 | - |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S.213.5 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pTyr701 of human STAT1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 59 µg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_10983352 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with the corresponding phospho-tyrosines of other STAT proteins.
Target Information
STAT1 (Signal Transducers and Activators of Transcription 1) is a member of the STAT family of transcription factors.
It can be activated by interferon-alpha, interferon-gamma, EGF, PDGF, and IL6, regulating genes involved in cell growth, apoptosis, immune responses, and lipid metabolism.
STAT1 plays a crucial role in mediating cell viability in response to various stimuli and pathogen exposure.
The STAT1 gene is located on chromosome 2 and is activated by extracellular signaling polypeptides such as cytokines, interferons, and growth factors.
After phosphorylation by JAK tyrosine kinases, STAT1 enters the nucleus to regulate transcription of multiple genes.
Among the seven STAT types, STAT1, STAT3, STAT5a, and STAT5b have broad activation profiles.
STAT1 is activated by ligands including the IFN family (IFN-Alpha, IFN-Beta, IFN-Gamma, IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, G-CSF), and receptor tyrosine kinases (EGF, PDGF, CSF-1).
Two alternatively spliced transcript variants encoding distinct isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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