Thermo Fisher Scientific IRS1 Monoclonal Antibody (J.566.7)
IRS1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체로, Western blot에 최적화되어 있습니다. 인간, 마우스, 랫 등 다양한 종에 반응하며, 고순도 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며 -20°C에서 보관합니다.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J.566.7 |
| Immunogen | Synthetic peptide corresponding to the sequence surrounding Ser612 of human IRS-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 708 µg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100 µg/mL BSA, 50% glycerol |
| Contains | <0.02% sodium azide |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_11000734 |
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
IRS-1 is a major substrate of the insulin receptor and is phosphorylated in response to stimulation by insulin, IGF-1, and IL-4. It undergoes phosphorylation on serine, threonine, and tyrosine residues across various tissues. Casein kinase II mediates a portion of the insulin-stimulated phosphorylation of IRS-1, particularly at Thr502 and Ser99. IRS-1 also contains three putative 14-3-3 binding sites (Ser270, Ser374, Ser641), with the Ser270 motif located in the phosphotyrosine-binding domain responsible for insulin receptor interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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