Thermo Fisher Scientific ZBP1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-2.0 µg/mL

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Immunohistochemistry (IHC)

20 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

2.5 µg/mL

-

Immunoprecipitation (IP)

-

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Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

An 18 amino acid synthetic peptide from near the carboxy terminus of human ZBP1. The immunogen is located within the last 50 amino acids of ZBP1. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ZBP1,uniProtId:Q9H171-1,ncbiNodeId:9606,antigenRange:429,antigenLength:429,antigenImageFileName:PA5-20455_ZBP1_Q9H171-1_Rabbit.svg,antigenImageFileNamePDP:PA5-20455_ZBP1_Q9H171-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154181

Product Specific Information

Suggested positive controls are mouse small intestine or human small intestine tissue lysate.

PA5-20455 can be used with blocking peptide PEP-0572.

PA5-20455 detects ZBP1 in WB application with human samples.

Target Information

Z-DNA binding protein 1 (ZBP1) belongs to a family of proteins that contain the Zalpha domain which binds specifically to left-handed Z-DNA and Z-RNA. ZBP1 was initially identified as a novel gene that was up-regulated in activated macrophages in mice bearing ascites tumors, suggesting that is may play a role in processes such as host response in neoplasia. More recent reports indicate that the cytosolic ZBP1 has can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of ZBP1 to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK). Multiple isoforms of ZBP1 are known to exist.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.