Thermo Fisher Scientific EN2 Monoclonal Antibody (1E1)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

Immunocytochemistry (ICC/IF)

40 µg/mL

-

ELISA (ELISA)

3 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

1E1

Immunogen

EN2 (NP_001418.2, 86 a.a. approximately 210 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EN2,uniProtId:P19622-1,ncbiNodeId:9606,antigenRange:86-210,antigenLength:333,antigenImageFileName:H00002020-M03_EN2_P19622-1_House_mouse.svg,antigenImageFileNamePDP:H00002020-M03_EN2_P19622-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: GTCCAGAGGG RGGGAGGEGG ASGAEGGGGA GGSEQLLGSG SREPRQNPPC APGAGGPLPA AGSDSPGDGE GGSKTLSLHG GAKKGGDPGG PLDGSLKARG LGGGDLSVSS DSDSSQAGAN LGAQP

Target Information

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the engrailed (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.