Thermo Fisher Scientific Phospho-Aurora Kinase B (Thr232) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500- 1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

ELISA (ELISA)

1:10,000-1:30,000

-

Immunoprecipitation (IP)

1:250-1:2,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region surrounding T232 of Human Aurora Kinase B protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.87 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Anti-Phospho Aurora B pT232 affinity purified antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. Reactivity occurs against human Aurora Kinase B pT232 protein and the antibody is specific for the phosphorylated form of the protein. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. However, 100% sequence homology as indicated by BLAST analysis is on record for this protein from human, mouse, rat, cow, pig, dog and chimpanzee. Cross reactivity with Aurora Kinase B from other sources is not known.

Target Information

The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.