Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet 395, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet 395, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (1.0 µg)/test

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 563
• Brilliant UV 661
• Brilliant UV 737
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 711
• Brilliant Violet 786
• FITC
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 700
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Request custom conjugation available

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor acting as a receptor for CCL19 and CCL21. CCR7 is expressed on T cells and dendritic cells (DCs) and is involved in cell migration, chemotaxis, and maturation processes.
For optimal staining, incubation time should be at least 45 minutes at 2–8°C.
This antibody is pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells at 5 µL (1.0 µg) per test.
CCR7 expression helps distinguish naive, central, and effector memory T cell populations.

Fluorochrome Information:
Brilliant Ultra Violet™ 395 (BUV395) emits at 395 nm and is designed for cytometers equipped with a 355 nm UV laser.
Excitation: 350 nm; Emission: 395 nm; Laser: Ultraviolet Laser.

When using multiple polymer dye-conjugated antibodies (Super Bright™, Brilliant Violet™, Brilliant Ultra Violet™), use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific interactions.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

Target Information

CCR7 belongs to the G protein-coupled receptor family and is induced by Epstein-Barr virus (EBV). It is expressed in blood, bone marrow, lymph node, and intestine, particularly in activated B and T lymphocytes. CCR7 regulates memory T cell migration to inflamed tissues and dendritic cell maturation.
Its specific ligands include chemokine (C-C motif) ligand 19 (CCL19/ECL).
C-C chemokine receptors such as CCR1–CCR5 are key mediators of T cell chemotaxis and phagocyte migration during inflammation.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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