Thermo Fisher Scientific SRp75 Polyclonal Antibody
SRp75 단백질을 인식하는 Thermo Fisher Scientific의 토끼 폴리클로날 항체. Western blot, IHC, IP 등 다양한 응용에 적합. 액상 형태로 제공되며 4°C에서 보관. FFPE 조직의 IHC 시 citrate buffer(pH 6.0) 전처리 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 | — |
| Immunohistochemistry (IHC) | 1:500–1:2,000 | — |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 | — |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate | — |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 250 and 300 of Human Pre-mRNA-Splicing Factor SRP75 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene. SRp75 belongs to the arginine/serine-rich factor family and likely plays a role in alternative mRNA splicing.
The SR factor family contains one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain. They are essential for constitutive splicing and regulate alternative splicing in a concentration-dependent manner.
Most SR proteins, including SC35 and SRp40, are confined to the nucleus, while SF2/ASF, SRp20, and 9G8 shuttle between the nucleus and cytoplasm, contributing to mRNA transport.
The activity of SR proteins in regulated splicing is antagonized by hnRNP A/B family proteins, which can alter splice site selection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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