Thermo Fisher Scientific SRp75 Polyclonal Antibody
Thermo Fisher Scientific의 SRp75 Polyclonal Antibody는 인간 SRSF4 단백질을 인식하는 토끼 IgG 항체로, Western blot, IHC, IP 등에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 액상 형태로 1 mg/mL 농도입니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific SRp75 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 250 and 300 of Human Pre-mRNA-Splicing Factor SRP75 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | Phosphate/tris citrate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene.
SRp75 belongs to the arginine/serine-rich factor family and plays a role in alternative mRNA splicing.
SR factors contain RNA recognition motifs (RRM) and an RS-rich domain, essential for constitutive and regulated splicing.
Most SR proteins, including SC35 and SRp40, are localized in the nucleus, while SF2/ASF, SRp20, and 9G8 shuttle between the nucleus and cytoplasm, contributing to mRNA transport.
SR protein activity in splicing regulation is antagonized by hnRNP A/B proteins, which influence splice site selection.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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