Thermo Fisher Scientific SRp75 Polyclonal Antibody
Rabbit polyclonal antibody targeting human SRp75 (SRSF4). Validated for WB, IHC, IHC-P, and IP applications. Liquid form, unconjugated, 1 mg/mL concentration. Recommended storage at 4°C, shipped on wet ice. For research use only.
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Thermo Fisher Scientific SRp75 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 400 and 450 of Human Pre-mRNA-Splicing Factor SRP75 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
Serine/arginine-rich protein 75 (SRp75) is encoded by the SRSF4 gene. SRp75 belongs to the arginine/serine-rich factor family and plays a role in alternative mRNA splicing.
SR factors contain one or more RNA recognition motifs (RRM) and an arginine/serine (RS)-rich domain. They are essential for constitutive splicing and regulate alternative splicing by influencing splice site selection.
Most SR proteins, including SC35 and SRp40, are localized in the nucleus, while SF2/ASF, SRp20, and 9G8 shuttle between the nucleus and cytoplasm, contributing to mRNA transport.
SR protein activity in regulated splicing is antagonized by hnRNP A/B family members, which induce shifts in splice site selection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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