Thermo Fisher Scientific OTUB1 Monoclonal Antibody (OTI5B8), TrueMAB
OTUB1 단백질을 인식하는 Mouse 모노클로날 항체로 Western blot, IHC, Flow cytometry에 사용 가능. HEK293T 세포에서 발현된 full-length human OTUB1을 면역원으로 제작. Lyophilized 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. 연구용 전용 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5B8 |
| Immunogen | Full length human recombinant protein of human OTUB1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
OTUB1 is a member of the OTU superfamily of predicted cysteine proteases. It functions as a highly specific ubiquitin iso-peptidase, cleaving ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with other ubiquitin proteases and E3 ubiquitin ligases that regulate cytokine gene transcription in the immune system. OTUB1 likely participates in ubiquitin-dependent pathways by providing an editing function for polyubiquitin chain growth. Multiple transcript variants exist due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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