Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Rabbit Polyclonal 항체로, Western blot 및 Immunocytochemistry에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 기반 완충액에 보관됩니다. 세포주기 조절 및 암 연구용으로 활용 가능합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human E2F1. Recombinant protein control fragment (Product #RP-104601) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.3 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2790259 |
Product Specific Information
Immunogen sequence:
PDLLLFATPQ APRPTPSAPR PALGRPPVKR RLDLETDHQY LAESSGPARG RGRHPGKGVK SPGEKSRYET
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain (acidic amino acids enriched)
- Tumor suppressor protein association domain (embedded within the transactivation domain)
E2F1, along with E2F2 and E2F3, also contains a cyclin binding domain. This protein binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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