Thermo Fisher Scientific E2F3 Polyclonal Antibody
Rabbit polyclonal antibody against human E2F3, validated for ICC/IF. Recognizes recombinant E2F3 protein. Supplied as liquid, unconjugated, 0.05 mg/mL in PBS with 40% glycerol. Suitable for research use in cell cycle and transcription studies.
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Applications
Immunocytochemistry (ICC/IF)
- Tested dilution: 0.25–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human E2F3. Recombinant protein control fragment (Product # RP-108327) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2790262 |
Product Specific Information
Immunogen sequence:
MRKGIQPALE QYLVTAGGGE GAAVVAAAAA ASMDKRALLA SPGFAAAAAA AAAPGAYIQI LTTNTSTTSC SSSLQ
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain (within transactivation domain)
E2F3, along with E2F1 and E2F2, possesses an additional cyclin binding domain and binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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