Thermo Fisher Scientific B-Myb Polyclonal Antibody
인간 B-Myb 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 액상형 1 mg/mL 농도. FFPE 조직에서는 citrate buffer pH 6.0을 이용한 epitope retrieval 권장.
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Thermo Fisher Scientific B-Myb Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 450 and 500 of human MYB-related protein B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Nucleocytoplasmic transport is a signal- and energy-dependent process occurring through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits (karyopherins). Importin alpha binds the NLS-containing cargo in the cytoplasm, and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP-binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein, while importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential for translocation through the pore complex. The protein encoded by this gene belongs to the importin beta family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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