Thermo Fisher Scientific B-Myb Polyclonal Antibody
Thermo Fisher Scientific의 B-Myb Polyclonal Antibody는 인간 B-Myb 단백질(250–300번 잔기)을 인식하는 토끼 IgG 폴리클로날 항체입니다. WB, IHC(P), IP에 사용 가능하며 항원 친화 크로마토그래피로 정제되었습니다. 액상 형태로 4°C에서 보관하며 연구용으로 적합합니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 250 and 300 of human MYB-related protein B |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | Tris citrate/phosphate, pH 7–8 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Nucleocytoplasmic transport is a signal- and energy-dependent process that occurs through nuclear pore complexes embedded in the nuclear envelope. Import of proteins containing a nuclear localization signal requires the NLS import receptor, a heterodimer of importin alpha and beta subunits (karyopherins). Importin alpha binds the NLS-containing cargo in the cytoplasm, while importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP-binding protein Ran, the complex moves into the nuclear pore complex and dissociates. Importin alpha enters the nucleoplasm with its passenger protein; importin beta remains at the pore. Interactions between importin beta and FG repeats of nucleoporins are essential for translocation through the pore complex. The encoded protein belongs to the importin beta family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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