Thermo Fisher Scientific CD23 Monoclonal Antibody (B3B4), NovaFluor Red 725, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.4 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Biotin
• Brilliant UV 805
• eFluor 450
• eFluor 660
• FITC
• NovaFluor Blue 510
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 710
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 755
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation available

Product Specific Information

The B3B4 monoclonal antibody reacts with mouse CD23, a 45 kDa type II transmembrane glycoprotein.
CD23 is expressed on resting conventional B cells and modulated upon B-cell activation. B-1 lineage (CD5+ B cells) does not express CD23. Soluble CD23 forms are biologically active and function as a low-affinity receptor for IgE, regulating IgE response and B-cell activation. CD21 is known to bind CD23.

Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse splenocytes using ≤0.4 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Antibody titration is recommended for optimal performance.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Usage Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability assessment.
• Add 5 µL of CellBlox Plus Blocking Buffer per sample (regardless of number of NovaFluors) before combining with cells.
• For single-color controls: use 5 µL buffer per 100 µL cell sample (10³–10⁸ cells).

Technology:
NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures, enabling optimized fluorescent signatures with reduced spillover and spread.

Excitation: 636 nm
Emission: 727 nm
Laser: 633–640 nm (Red)

Target Information

CD23 is a 45 kDa glycoprotein expressed on a subset of peripheral blood and tonsil B cells, and strongly expressed on EBV-transformed B lymphoblasts. It serves as the low-affinity IgE receptor and is involved in B-cell growth, differentiation, and IgE production. Soluble CD23 acts as a potent mitogenic factor.
CD23 dysfunction is associated with diseases such as chronic conjunctivitis and chronic lymphocytic leukemia.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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