Thermo Fisher Scientific Click-iT EdU Alexa Fluor 488 HCS Assay, 2 x 96 well plates

Invitrogen™ Click-iT™ EdU Alexa Fluor™ 488 HCS Assay

The Click-iT™ EdU Alexa Fluor™ 488 HCS Assay is a superior alternative to traditional proliferation assays optimized for high-content imaging applications.
In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye through a fast, highly-specific click reaction.
This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

주요 특징

• Simple — Works the first time, every time, in less time
• Efficient — No denaturation steps or harsh treatment required
• Gentle on samples — Better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent — Not dependent on variable antibody lots for detection

Superior Proliferation Methodology

The Click-iT™ EdU Alexa Fluor™ 488 HCS Assay provides a superior alternative to BrdU assays for measuring cell proliferation.
EdU (5-ethynyl-2`-deoxyuridine) is a nucleoside analog of thymidine incorporated into DNA during active synthesis.
Mild fixation and detergent permeabilization allow the small molecule-based Click-iT™ detection reagent to access DNA easily, enabling accurate and content-rich results compatible with cell cycle analysis and other intracellular or extracellular targets.

The kit is optimized for high-content imaging applications.
Visit the Click-iT™ technology area of our website for kits designed for fluorescence microscopy, microplate, or flow cytometry platforms.

Notes

• The Click-iT™ assay can be used on cells in culture or in vivo following administration of EdU by feeding or injection.
• Dual pulse experiments with BrdU are possible using the anti-BrdU (clone MoBu-1) antibody, which does not cross-react with EdU.
• Compatible with immunohistochemical, immunocytochemical, and fluorescent dyes tolerant to fixation or designed for fixed-cell labeling.

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