Thermo Fisher Scientific Click-iT EdU Alexa Fluor 555 HCS Assay, 2 x 96 well plates

Invitrogen™ Click-iT™ EdU Alexa Fluor™ 555 HCS Assay

The Click-iT™ EdU Alexa Fluor™ 555 HCS Assay is a superior alternative to traditional proliferation assays optimized for high-content imaging applications.
In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ 555 dye through a fast, highly specific click reaction.
This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

주요 특징

• Simple — Works the first time, every time, in less time
• Efficient — No denaturation steps or harsh treatment required
• Gentle on samples — Better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent — Not dependent on variable antibody lots for detection

Superior Proliferation Methodology

The Click-iT™ EdU Alexa Fluor™ 555 HCS Assay provides a superior alternative to BrdU assays for measuring cell proliferation.
EdU (5-ethynyl-2'-deoxyuridine) is a nucleoside analog of thymidine incorporated into DNA during active synthesis.
With Click-iT™ EdU, mild fixation and detergent permeabilization allow the small molecule-based detection reagent to access DNA easily.
As a result, the assay is easy to use, accurate, and compatible with cell cycle analysis and other intracellular or extracellular labeling for truly content-rich results.

The kit is optimized for high-content imaging applications.
Visit the Click-iT™ technology area of the Thermo Fisher Scientific website for kits designed for fluorescence microscopy, microplate, or flow cytometry platforms.

Notes

• The Click-iT™ assay can be used on cultured cells or in vivo following administration of EdU by feeding or injection.
• It can be used with BrdU in dual pulse experiments using the anti-BrdU (clone MoBu-1) antibody, which does not cross-react with EdU.
• Compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation-tolerant or designed for fixed-cell labeling.

제품 사양

제품 이미지