Thermo Fisher Scientific Click-iT EdU Alexa Fluor 647 HCS Assay, 2 x 96 well plates

Invitrogen™ Click-iT™ EdU Alexa Fluor™ 647 HCS Assay

The Click-iT™ EdU Alexa Fluor™ 647 HCS Assay is a superior alternative to traditional proliferation assays optimized for high-content imaging applications.
In this assay, the modified thymidine analogue EdU is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly-specific click reaction.
This fluorescent labeling of proliferating cells is accurate and compatible with antibody methods due to the mild click protocol.

주요 특징

• Simple — Works the first time, every time, in less time
• Efficient — No denaturation steps or harsh treatment required
• Content-rich results — Better preservation of cell morphology, antigen structure, and DNA integrity
• Consistent — Not dependent on variable antibody lots for detection

Superior Proliferation Methodology

The Click-iT™ EdU Alexa Fluor™ 647 HCS Assay provides a superior alternative to BrdU assays for measuring cell proliferation.
EdU (5-ethynyl-2`-deoxyuridine) is a nucleoside analog of thymidine and is incorporated into DNA during active DNA synthesis.
With Click-iT™ EdU, mild fixation and detergent permeabilization allow the small molecule-based detection reagent to access the DNA easily.
As a result, the assay is not only easy to use but also more accurate and compatible with cell cycle analysis and other intracellular or extracellular targets for truly content-rich results.

The kit is optimized for high-content imaging applications. Visit the Click-iT™ technology area of our website for kits designed for fluorescence microscopy, microplate, or flow cytometry platforms.

Learn more about Click-iT™ technology

Notes

• The Click-iT™ assay can be used on cells in culture or in vivo following administration of EdU by feeding or injection methods.
• The assay can be used with BrdU in dual pulse experiments using the anti-BrdU (clone MoBu-1) antibody, which does not cross-react with EdU.
• Compatible with immunohistochemical, immunocytochemical, and fluorescent dyes that are fixation tolerant or designed for fixed-cell labeling.

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