Thermo Fisher Scientific TRPM7 Monoclonal Antibody (S74), PE

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

S74

Immunogen

Fusion protein amino acids 1817-1863 (C- terminus) of mouse TrpM7 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TRPM7,uniProtId:Q923J1-1,ncbiNodeId:10090,antigenRange:1817-1863,antigenLength:1863,antigenImageFileName:MA5-45426_TRPM7_Q923J1-1_House_mouse.svg,antigenImageFileNamePDP:MA5-45426_TRPM7_Q923J1-1_House_mouse_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

PE PE PE

View additional formats

• Unconjugated
• APC
• FITC
• PerCP
• Request custom conjugation

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931880

Product Specific Information

1 µg/mL of MA5-45426 was sufficient for detection of TrpM7 in 10 µg of COS cell lysate transiently transfected with TprM7 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 220kDa in extracts of cells transiently transfected with TrpM7. No cross-reactivity against TrpM6.

Target Information

TRPCs, mammalian homologs of the Drosophila transient receptor potential (trp) protein, are ion channels that are thought to mediate capacitative calcium entry into the cell. TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.