Thermo Fisher Scientific TRPM7 Monoclonal Antibody (S74), APC
TRPM7 단백질을 검출하기 위한 마우스 단클론 항체로, APC 형광 표지되어 있습니다. Western blot, IHC, ICC 등 다양한 응용에 적합하며, 인간, 마우스, 랫트 반응성을 가집니다. 고순도 Protein G 정제, 4°C 보관, 연구용 전용 제품입니다.
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Thermo Fisher Scientific TRPM7 Monoclonal Antibody (S74), APC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:1,000 |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | S74 |
| Immunogen | Fusion protein amino acids 1817–1863 (C-terminus) of mouse TrpM7 |
| Conjugate | APC |
| Excitation/Emission Max | 651/660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931877 |
Available Formats
- Unconjugated (MA5-27620)
- FITC (MA5-45424)
- PE (MA5-45426)
- PerCP (MA5-45425)
- Custom conjugation available upon request
Product Specific Information
- 1 µg/mL of MA5-45423 detects TrpM7 in 10 µg of COS cell lysate transiently transfected with TrpM7 by colorimetric immunoblot using Goat anti-mouse IgG:HRP secondary antibody.
- Detects approximately 220 kDa band in TrpM7-transfected cells.
- No cross-reactivity with TrpM6.
Target Information
TRPM7 (Transient Receptor Potential Melastatin 7) is a unique protein combining ion channel and kinase functions. It mediates calcium and monovalent cation influx, contributing to cell depolarization and intracellular calcium increase. The kinase domain phosphorylates itself and other substrates, and its activity is essential for channel function, being ATP-dependent.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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