Thermo Fisher Scientific Phospho-Histone H3 (Thr3) Polyclonal Antibody
Western blot과 IHC(P)에서 사용 가능한 Rabbit Polyclonal 항체로, 인간 및 설치류 등 다양한 종에 반응. Histone H3 Thr3 인산화 부위를 특이적으로 인식하며 다른 변형 히스톤과 교차 반응 없음. 항원 친화 크로마토그래피로 정제된 액상 형태.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding pThr3 of human histone H3 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.01M HEPES, pH 7.5, with 0.15M NaCl, 50% glycerol, 100µg/mL BSA |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985541 |
Product Specific Information
- It is not recommended to aliquot this antibody.
- This antibody is not cross-reactive with other phosphorylated histones or with acetylated histones.
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome.
Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability.
Post-translationally, histones are modified in various ways to change chromatin structure or allow for binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the nucleosome core and undergoes modifications such as methylation, acetylation, and phosphorylation that influence cellular processes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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