Thermo Fisher Scientific CD100 Monoclonal Antibody (547412)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

2.5 µg per million cells

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

547412

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100 Phe24-Thr657 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD100 (SEMA4D),uniProtId:O09126-1,ncbiNodeId:10090,antigenRange:24-657,antigenLength:861,antigenImageFileName:MA5-24248_CD100_SEMA4D_O09126-1_Rat.svg,antigenImageFileNamePDP:MA5-24248_CD100_SEMA4D_O09126-1_Rat_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2610476

Product Specific Information

In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.

Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.

Target Information

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100 (also known as semaphorin 4d), a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.