Thermo Fisher Scientific CD100 Monoclonal Antibody (547412)
Flow Cytometry용 마우스 반응성 CD100 단일클론 항체로, Rat IgG2b 아이소타입입니다. 단백질 A/G로 정제된 동결건조 제형이며, PBS와 5% 트레할로스에 보관됩니다. CD100(Semaphorin 4D) 인식 항체로 B세포 신호 조절 연구에 적합합니다.
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Applications
Flow Cytometry (Flow)
- Tested Dilution: 2.5 µg per million cells
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 547412 |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100 (Phe24–Thr657) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2610476 |
Product Specific Information
- In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.
- Reconstitute in sterile PBS to a final concentration of 0.5 mg/mL.
Target Information
Semaphorins are neuronal chemorepellants that guide neuronal development.
CD100 (Semaphorin 4D) is a 150 kDa homodimer that induces B cell aggregation and enhances viability in vitro.
It modulates CD40–CD40L signaling by promoting B cell aggregation and survival while downregulating CD23 expression.
CD72 serves as a receptor for CD100, regulating B cell signaling.
CD100 is expressed on resting and PHA-stimulated T cells, weakly on NK cells, EBV-transformed B cells, monocytes, and tumor/peripheral blood T cell lines, and more strongly on activated T and B cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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