Thermo Fisher Scientific Laminin alpha-3 Monoclonal Antibody (546215)

Applications

Tested Dilution

Publications

Western Blot (WB)

2 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

8-25 µg/mL

-

Flow Cytometry (Flow)

0.25 µg/10^6 cells

-

Immunoprecipitation (IP)

25 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

546215

Immunogen

CHO-derived recombinant human Laminin alpha 3/Laminin-5 (aa 21-1713) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Laminin alpha-3,uniProtId:Q16787-2,ncbiNodeId:9606,antigenRange:21-1713,antigenLength:3333,antigenImageFileName:MA5-24246_Laminin_alpha-3_Q16787-2_House_mouse.svg,antigenImageFileNamePDP:MA5-24246_Laminin_alpha-3_Q16787-2_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2607668

Product Specific Information

In direct ELISAs, no cross reactivity with recombinant human Laminin alpha 1, beta 1, gamma 2, or recombinant mouse Laminin alpha 4 is observed.

Reconstitute at 0.5 mg/mL in sterile PBS.

Target Information

Laminin is a non-collagenous protein of basement membranes. By rotary shadowing it has a cross-like shape, consisting of one long arm, (400 kD) and three short arms (200 kD). Antibodies to laminin localize exclusively in basement membranes. Laminin is found throughout the basement membrane, but the reaction with the antibodies to laminin is more intense in the lamina lucida, the part of the basement membrane adjacent to the cell membrane. Laminin binds to type IV collagen, a component exclusively localized in the basement membrane. Laminin has been shown to play a role in cell adhesion and attachment in vivo and in vitro.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.