Thermo Fisher Scientific Laminin alpha-3 Monoclonal Antibody (546215)
Laminin alpha-3 단백질을 인식하는 Mouse monoclonal 항체로, Western blot, IHC, ICC, Flow cytometry, IP에 사용 가능. 인간 시료에 반응하며 PBS buffer에 동결건조 형태로 제공. 교차 반응 없음, 세포 부착 및 기저막 연구에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5 µg/mL |
| Immunocytochemistry (ICC/IF) | 8–25 µg/mL |
| Flow Cytometry (Flow) | 0.25 µg/10^6 cells |
| Immunoprecipitation (IP) | 25 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 546215 |
| Immunogen | CHO-derived recombinant human Laminin alpha 3/Laminin-5 (aa 21–1713) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.5 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No Preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607668 |
Product Specific Information
- In direct ELISAs, no cross-reactivity with recombinant human Laminin alpha 1, beta 1, gamma 2, or recombinant mouse Laminin alpha 4 is observed.
- Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
Laminin is a non-collagenous protein of basement membranes. It has a cross-like shape consisting of one long arm (400 kD) and three short arms (200 kD). Antibodies to laminin localize exclusively in basement membranes, with stronger reactivity in the lamina lucida adjacent to the cell membrane. Laminin binds to type IV collagen and plays a role in cell adhesion and attachment both in vivo and in vitro.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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