Thermo Fisher Scientific Glypican-3 (GPC3) Recombinant Mouse Monoclonal Antibody (rGPC3, 863)
Glypican-3(GPC3) 단백질을 인식하는 Thermo Fisher Scientific의 재조합 마우스 단클론 항체. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG1, kappa 형태로 정제된 고순도 항체. 연구용으로만 사용 가능.
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Thermo Fisher Scientific Glypican-3 (GPC3) Recombinant Mouse Monoclonal Antibody (rGPC3, 863)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rGPC3, 863 |
| Immunogen | Recombinant full-length human GPC3 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
GPC3 is a cell surface proteoglycan that bears heparan sulfate. It may be involved in growth modulation in mesodermal tissues and organs and in the regulation of IGF2 receptor interactions. Members of the glypican family are anchored to the membrane via GPI linkage and play roles in cell division, growth regulation, and tumor predisposition.
Deletion mutations in GPC3 cause Simpson-Golabi-Behmel syndrome (SGBS), characterized by pre- and postnatal overgrowth with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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