
Thermo Fisher Scientific Glypican-3 (GPC3) Recombinant Mouse Monoclonal Antibody (rGPC3, 863)
Glypican-3(GPC3)을 인식하는 mouse recombinant monoclonal antibody로, IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 사용 가능. Human 및 Rat 반응성. Protein A 정제, 보존제 없음. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | rGPC3, 863 |
| Immunogen | Recombinant full-length human GPC3 protein (amino acids 1–580, UniProt ID: P51654-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | –20°C or –80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry on PFA-fixed tissues: incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
GPC3 (Glypican-3) is a cell surface heparan sulfate proteoglycan involved in growth modulation in mesodermal tissues and organs. It may regulate IGF2 interactions with its receptor and influence cell division and tumor predisposition.
Mutations in GPC3 cause Simpson-Golabi-Behmel syndrome (SGBS), characterized by pre- and postnatal overgrowth with visceral and skeletal anomalies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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