
Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
DyLight 650 형광 표지된 Donkey anti-Rat IgG (H+L) 교차흡착 2차 항체로, Rat IgG 검출용으로 높은 특이성과 낮은 교차반응성을 제공합니다. WB, IHC, ICC, Flow Cytometry 등 다양한 응용에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다.
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Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 650
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS (pH 6.8–7.4) with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556609 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, human, mouse, rabbit, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, goat, human, mouse, rabbit, and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in detection, sorting, or purification of the specified target.
Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, fragmentation, conjugation) to yield highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 680
- DyLight 755
- DyLight 800
- Custom conjugation available upon request
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