Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 680
Rat IgG 검출용 DyLight 680 결합 Donkey anti-Rat IgG (H+L) 교차흡착 2차 항체로, WB, IHC, ICC, Flow 등 다양한 응용에 사용 가능. 높은 특이성과 낮은 교차반응성 제공. 항원 친화 크로마토그래피로 정제된 액상 항체.
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Thermo Fisher Scientific Donkey anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, DyLight 680
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:20,000 |
| Immunohistochemistry (IHC) | 1:50–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:500 |
| Flow Cytometry (Flow) | 1:50–1:200 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Rat |
| Host / Isotype | Donkey / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Rat IgG-heavy and light chain |
| Conjugate | DyLight™ 680 |
| Excitation / Emission Max | 676 / 705 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2556610 |
| Target | IgG |
| Cross Adsorption | Against bovine, chicken, goat, human, mouse, rabbit, and sheep |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody is cross-adsorbed and exhibits minimal reactivity to bovine, chicken, goat, human, mouse, rabbit, and sheep.
Target Information
Anti-Rat secondary antibodies are affinity-purified and exhibit well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of rat IgG.
Secondary antibodies enable versatility across detection systems (HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
Typically, these antibodies are produced by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- DyLight 488
- DyLight 550
- DyLight 594
- DyLight 650
- DyLight 755
- DyLight 800
- Custom conjugation available upon request
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