Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Super Bright 600, eBioscience
인간 CD20 항원을 인식하는 2H7 클론 단일클론 항체로, Super Bright 600 형광체가 결합되어 있습니다. Flow cytometry에 최적화되어 있으며, B세포 활성화 연구에 적합합니다. 광민감성이 있어 암실 보관이 필요합니다.
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Thermo Fisher Scientific CD20 Monoclonal Antibody (2H7), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 6 publications |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host/Isotype | Mouse / IgG2b, kappa |
| Recommended Isotype Control | Mouse IgG2b kappa Isotype Control (eBMG2b), Super Bright™ 600 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2H7 |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414/601 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2688265 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395, 496, 615, 737, 805
- Brilliant Violet 421, 605, 650, 711
- eFluor 450, 506
- FITC
- NovaFluor Blue 610-70S, 660-120S
- NovaFluor Red 710, 725
- NovaFluor Yellow 590, 660
- PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The 2H7 monoclonal antibody reacts with human CD20, a 33–36 kDa transmembrane protein expressed on developing and mature B cells, but not plasma cells. CD20 plays a role in B-cell activation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated for flow cytometry of human peripheral blood cells at 5 µL (0.5 µg) per test
- Recommended Cell Range: 10⁵–10⁸ cells/test
- Excitation/Emission: 405 nm / 600 nm (Violet laser)
- Filter Recommendation: 610/20 bandpass filter
Super Bright 600 is a tandem dye sensitive to photo-induced oxidation; protect from light.
When using multiple Super Bright dyes, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
For fixation, samples may be stored in IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD20 is a non-glycosylated surface phosphoprotein (33–37 kDa) expressed on mature and malignant B cells, a subset of T lymphocytes, and follicular dendritic cells. It regulates calcium conductance, cell cycle progression, and B-cell proliferation. CD20 remains on the cell surface after antibody binding, making it a valuable target for B-cell depletion therapies. Disorders associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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