Thermo Fisher Scientific Sheep anti-Rabbit IgG (H+L) Secondary Antibody, HRP
HRP 결합형 양 항-토끼 IgG 2차 항체로 Western blot, IHC, ELISA에 적합. 고감도 및 높은 특이성 제공. 친화 크로마토그래피로 정제된 폴리클로날 항체. 연구용으로만 사용.
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Thermo Fisher Scientific Sheep anti-Rabbit IgG (H+L) Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534843 |
| Target | IgG |
| Antibody Form | Whole Antibody |
Product Specific Information
- Each lot’s sensitivity is confirmed using ELISA.
- Specificity verified by immunoelectrophoresis (IEP).
- Rehydrate with 1.1 mL deionized water, stand 30 minutes at room temperature to dissolve.
- Centrifuge to remove particulates and prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on IEP, no reactivity is observed to non-immunoglobulin rabbit serum proteins.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified with well-characterized specificity for rabbit immunoglobulins. They are useful for detection, sorting, or purification of rabbit IgG targets.
Secondary antibodies increase versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody.
These antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
Additional Formats
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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