Thermo Fisher Scientific Rabbit anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, TRITC
TRITC로 결합된 Rabbit anti-Mouse IgG (H+L) 2차 항체로, 면역세포염색(ICC/IF)에 적합합니다. Mouse IgG에 특이적이며, 사람 단백질에 대한 교차 반응이 최소화되어 있습니다. 552/578 nm 형광 특성을 가지며, 고감도 신호 검출에 활용됩니다.
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Applications
- Immunocytochemistry (ICC/IF): 4 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobulins Heavy and Light chains |
| Conjugate | TRITC |
| Excitation / Emission Max | 552 / 578 nm |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534842 |
| Target | IgG |
| Cross Adsorption | Against human serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
- Product has been overfilled to ensure complete recovery.
- Centrifuge to remove particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to non-immunoglobulin mouse serum proteins or human serum proteins.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of target proteins.
Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and increase sensitivity through signal amplification.
Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Additional Formats Available
- Alexa Fluor 350
- Alexa Fluor 488
- Alexa Fluor 555
- Alexa Fluor 568
- Alexa Fluor 594
- Alexa Fluor 633
- Alexa Fluor 647
- Alexa Fluor 680
- AP
- Biotin
- DyLight 488
- DyLight 550
- DyLight 650
- DyLight 680
- DyLight 755
- DyLight 800
- FITC
- HRP
- Custom conjugation available
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배송 정보
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